Prerequisites

This tutorial aims to help identify cell populations from single cell transcriptomics data.

It is composed of R code that you can execute in a R console. You can also use any R graphical environment like Rstudio. You can retrieve the associated Rscript or R Markdown file (An executable document that combine text and code to produce formatted report) here.

Installation of R and RStudio

Here is some documentation to help you install R and Rstudio if needed :

• https://larmarange.github.io/analyse-R/installation-de-R-et-RStudio.html
• https://bookdown.org/introrbook/intro2r/installing-r-and-rstudio.html

However you are provided with an Rstudio-server account to follow the IOC !

Technical prerequisites

The following points are required to be able to perform the practical parts of this tutorial correctly :

• Basic knowledge in R because there is no focus on R code
  • How to manipulate R objets (vectors, dataframe, lists)
  • Create basics functions
  • How to use lapply and mapply
• R version >= 4 installed
  • You can see all mandatory packages and their version at references with the sessionInfo