Deep Sequencing Applications
High throughput sequencing of DNA or RNA provides Qualitative (sequence) and Quantitative (number of reads) information.¶
- Whole Genome sequencing (WGS)
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- De novo sequence assembly for new genomes
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- Re-sequencing indel, mutation, snp identification and analysis
- Exome sequencing
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- capture probe to sequence only exons
- RADseq, ddRADseq
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- Sampling of genome to be sequenced
- Meta-genome sequencing
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- Pathogen / symbiotic flora identification or analysis
- ChIPseq (Chromatin Immunoprecipitation sequencing)
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- Epigenetic landscape, Transcription factor binding sites
- ATACseq (Assay for Transposase-Accessible Chromatin)
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- DNA accessibility (chromatin compaction, transcription factors…)
- 3C-seq (Chromosome conformation capture), 4C, 5C, ChIA-PET, Hi-C
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- mapping of long-range genomic interactions
- Medip-seq
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- DNA methylation studies
- RNA or small RNA sequencing
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- Transcriptome
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- Small or long non-coding RNA profiling
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- Single cell transcriptome analysis
- RIP-seq (RNA immunoprecipitation sequencing)
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- Protein associated RNAs
- CLIP-seq (Cross-linking immunoprecipitation sequencing)
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- Protein associated RNA duplexes