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Sequencing Protocol

DNA libraries and sequencing protocol for patient samples

  • Bone marrow samples from patients with Acute Lymphoid Leukemia (ALL)
  • Genomic DNA from bone marrow cells
  • DNA fragmentation (expected ~200-500nt long)
  • Selection of DNA fragments with Agilent capture probes: 2 regions of several dozens of kb centered on BCR and ABL1
  • Library preparation
  • Sequencing of both ends of fragments: Paired-End sequencing (see figure below)