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Cluster Annotation and Visualisations

You did it ! You succeed to perform the different types of gene set annotations ! Even if, the dataset is a little complex, with the help of your results and the paper you can identify the associated cell type for your clusters. You can read the following pages to finish the IOC : Cluster annotation and Visualisations !!

Well Done !

Do it yourself!

Render your RMD/QMD

To complete this week you'll need to :

  • 1. Resume the information obtain in the clusterProfiler analyses (ORA + GSEA)
  • 2. Identify each cluster
  • 3. Set a new ident in your seurat object
  • 4. Use the function DotPlot to visualize the most representated markers for each cluster
  • 5. Have fun with the VlnPlot, FeaturePlot and DimPlot functions

IMPORTANT

Please note you must be explicative in your cutoff choices and provide detailed explanations for each step of your thought process In general, try to explain in your own words for each step of your analysis!

Add your RMD/QMD in your Trello card. You can also add the html version of your rapport.

Thank you for your attention and see you soon 👏 👏 👏