DNA libraries and sequencing protocol for patient samples
- Bone marrow samples from patients with Acute Lymphoid Leukemia (ALL)
- Genomic DNA from bone marrow cells
- DNA fragmentation (expected ~200-500nt long)
- Selection of DNA fragments : 2 regions of several dozens of kb centered on BCR and ABL1
- Library preparation
- Sequencing of both ends of fragments: Paired-End sequencing (see figure below)
