Read Coverage using the Bamcoverage tool
Select the tool bamCoverage generates a coverage bigWig file from a given BAM or CRAM file (Galaxy Version 3.1.2.0.0)
- BAM/CRAM file:
Dataset CollectionandMap with BWA-MEM on collection 3 (mapped reads in BAM format) - Bin size in bases:
100 - Scaling/Normalization method: Do not normalize or scale
- Coverage file format:
bigWig - Compute an exact scaling factor:
no - Region of the genome to limit the operation to:
chr9 - Show advanced options:
yes - Ignore missing data?:
yes
Other options unchanged
-
run the tool
-
rerun the tool paying attention to
- reselect BAM/CRAM file:
Dataset CollectionandMap with BWA-MEM on collection 3 (mapped reads in BAM format) -
- Region of the genome to limit the operation to:
chr22
- Region of the genome to limit the operation to:
- reselect BAM/CRAM file:
