Metavisitor is a user-friendly and adaptable software to provide biologists, clinical researchers and possibly diagnostic clinicians with the ability to robustly detect and reconstruct viral genomes from complex deep sequence datasets. A set of modular bioinformatic tools and workflows was implemented as the Metavisitor package in the Galaxy framework. Using the graphical Galaxy workflow editor, users with minimal computational skills can use existing Metavisitor workflows or adapt them to suit specific needs by adding or modifying analysis modules.
Quick Start
Users who want to use Metavisitor on the Galaxy Mississippi Server, or got already the Metavisitor suite of tools installed in their own Galaxy server, can jump to the next chapter Prepare input data histories.
Availability of Metavisitor tools and workflows
Metavisitor has been developed at the ARTbio platform. Its tools and workflows are primarily available in [GitHub] (https://github.com/ARTbio/tools-artbio).
Metavisitor tools developed by ARTbio in the ARTbio GitHub
yac_clipper
concatenate_multiple_datasets
msp_sr_bowtie
msp_fasta_tabular_converter
fetch_fasta_from_ncbi
msp_cap3
cherry_pick_fasta
msp_blastparser_and_hits
msp_oases
blast_to_scaffold
blastx_to_scaffold
msp_sr_readmap_and_size_histograms
.
Other tools from other developers are included the suite_metavisitor_1_2. Of course, these tools are not available on our GitHub repository, but are available from the main Galaxy toolshed:
name="sra_tools" owner="iuc"
name="get_orfs_or_cdss" owner="peterjc"
name="trinityrnaseq" owner="anmoljh"
name="bowtie2" owner="devteam"
name="fastx_trimmer" owner="devteam"
name="fastq_to_fasta" owner="devteam"
name="fasta_filter_by_length" owner="devteam"
name="regex_find_replace" owner="jjohnson"
name="khmer_normalize_by_median" owner="iuc"
Availability of Metavisitor tools and workflows for Galaxy instance administrators
All metavisitor tools are available in the suite_metavisitor_1_2
Galaxy Admin can just install this suite of tools by using the Search Tool Shed
menu in their Admin panel, searching for "metavisitor", and installing the suite_metavisitor_1_2
tool suite.
Admins can also install the tools from the metavisitor_workflows
repository, which will provide in addition the metavisitors workflows.
Availability of Metavisitors workflows for any Galaxy instance user.
We have deposited the Metavisitors workflows in the myexperiment server, where they are searchable with "metavisitor" keyword and can be downloaded and reuploaded to the Galaxy instance.
Starting a Metavisitor Galaxy server from scratch
In the next three chapters, we provide documentation on two methods to set up a Galaxy server instances from scratch with pre-installed Metavisitor tools and workflows:
- Based on GalaxyKickstarter: see Metavisitor with GalaxyKickstarter (Ansible)
- Based on Docker: see Metavitor with Docker